孕期、哺乳期暴露十溴联苯醚对子代大鼠免疫功能的影响

Impact of PBDE-209 exposure during pregnancy and lactation on immune function of offspring rats

目的针对环境污染中十溴联苯醚对母婴健康存在潜在危害日益受到重视,研究经口胃灌十溴联苯醚对子代大鼠免疫功能的影响。方法对母代孕期前后及哺乳期持续高剂量胃灌十溴联苯醚后,观察其子代大鼠免疫器官发育情况、T淋巴细胞亚群及T淋巴细胞增殖转化功能、NK细胞表面分子CD161表达及免疫球蛋白(IgM、IgG)等变化。结果实验组免疫器官质量与对照组相比有变化,其T淋巴细胞亚群CD3+、CD3+CD8+、CD3+CD4+及NK细胞表面分子CD161+百分比、CD4+/CD8+比值下降,CD4-CD8-百分比升高。实验组T淋巴细胞增殖转化功能下降不明显,其IgM含量明显低于对照组,IgG含量变化不明显。结论母代持续暴露高剂量十溴联苯醚后,对子代免疫功能存在一定的不利影响。

Objective To assess the potential hazard of environmental deca-brominated diphenyl ether （PBDE-209） exposure to the immune function of the offspring rats. Methods The parental Wistar rats were exposed to PBDE-209 administered intragastrically during pregnancy and lactation, and the development of the immune organs and changes in T lymphocyte subset and their proliferation, NK cell surface markers CD161 and serum immunoglobulins （IgM and IgG） were observed. Results Significant differences in the weight of the immune organs were noted between the exposure group and control group. In the exposure group, the percentage ofT lymphocyte subset CD3^＋, CD4^＋, CD8^＋, CD4^＋CD8^＋, the percentage of NK cell surface markers CD161 and CD4^＋/CD8^＋ ratio were lowered, while the percentage of CD4^-CD8^- cells increased. T lymphocyte subset proliferation in the exposure group did not show obviously changes, but compared with the control group, the IgM level in the exposure group was significantly lowered. No significant differences were observed in IgG levels between the exposure and control groups. Conclusion Continuous exposure to high-dose PBDE-209 in female rats during pregnancy and lactation results in possible adverse effect on the immune function of the offspring rats.