兔VX2肝癌模型制备的改进及其磁共振影像学表现

Modification of the method for preparing rabbit liver VX2 tumor model and its MRI findings

目的改进兔VX2肝癌模型的制作方法,使之更为简便、省时,同时探讨瘤灶的MRI表现及MRI在动态检测瘤灶中的作用。方法新西兰大白兔16只,随机分成4组(每组4只),在螺旋CT引导下,采用瘤块组织接种于兔肝脏左、右叶,接种后14、18、22、26d分别行MRI平扫、增强扫描及病理分析。结果16只实验兔发现瘤灶,成瘤率100%;瘤灶以种植后18￣22dMRI显示最清楚和典型,直径1￣2cm,平扫呈低或等信号,增强扫描瘤灶边缘明显强化,种植后超过22d的肿瘤中央坏死明显。结论螺旋CT引导下进行兔VX2肝癌模型的制作,使制作过程更为简便、省时;采用磁共振平扫及增强扫描对瘤灶进行动态检测、评价,可更为准确、及时地观察瘤灶的发生、发展等影像学改变。

Objective To improve the method for preparing rabbit VX2 liver tumor model and observe the magnetic resonance imaging （MRI） features of the implanted tumors. Methods Sixteen adult New Zealand white rabbits were assigned randomly into 4 equal groups, and VX2 tumor tissues were implanted into the right and left liver lobes with spiral CT guidance. Plain and contrast-enhanced MR scann and pathological analysis were performed in different stages （14, 18, 22 and 26 days） after tumor implantation. Results Tumor implantation was successful in all the rabbits, and 18 to 22 days after tumor implantation, the diameters of the tumor ranged fi＇om 1 to 2 cm, which allowed observation and study. In plain MR scans, lower or equivalent tumor signal in comparison with hepatic parenchyma was observed, and contrast-enhanced scans produced obvious enhancement of the tumor edges. At 22 days after tumor implantation, obvious necrosis was observed in the center of the tumor. Conclusion This method of preparing rabbit VX2 liver tumor model with spiral CT guidance is simple and convenient, and the tumors can be observed effectively with dynamic plain and contrast-enhanced MR scans.