灵芝多糖拮抗前列腺素E2对小鼠脾细胞IFN-γ和TNF-α mRNA表达的抑制作用

Ganoderma polysaccharides antagonize prostaglandin E2-induced suppression of murine splenocyte IFN-γ and TNF-α mRNA expression

目的观察灵芝多糖拮抗前列腺素E2(PGE2)对小鼠脾细胞干扰素γ(IFN-γ)和肿瘤坏死因子α(TNF-α)mRNA表达的抑制作用。方法混合淋巴细胞培养反应作为实验模型,半定量RT-PCR方法检测IFN-γ和TNF-αmRNA的表达水平。结果PGE2连续作用4h后,脾细胞IFN-γmRNA的表达与对照组比较受到不同程度的抑制,当PGE2浓度在10μmol/L以上时具有统计学意义(P<0.01)。固定PGE2浓度为20μmol/L,合用不同浓度的灵芝多糖,当灵芝多糖为100mg/L以上时可部分对抗PGE2的抑制作用。培养8h后,PGE2明显抑制TNF-αmRNA的表达,灵芝多糖为100mg/L以上时可部分对抗。结论灵芝多糖可部分拮抗PGE2对小鼠脾细胞IFN-γ和TNF-αmRNA表达的抑制作用。

Objective To determine if Ganoderma polysaccharides can antagonize prostaglandin E2 （PGE2）-induced suppression of murine splenocyte interferon γ （IFN-γ） and tumor necrosis factor α （TNF-α） mRNA expression. Methods Mixed lymphocyte culture reaction was used as the experimental model. The expressions levels of IFN-γ and TNF-α mRNA were measured by semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）. Results After the cultures were treated with PGE2 for 4 h, IFN-γ mRNA expression was reduced as compared with the control, which was especially obvious when PGE2 concentrations exceeded 10 μmol/L （P〈0.01）. Ganoderma polysaccharides above 100 mg/L showed partial antagonistic effect against the inhibition of IFN-γ by PGE2 at the fixed concentration of 20 μmol/L. Further studies indicated that PGE2 （20 μmol/L） impaired the expression of TNF-α mRNA after an 8-hottr incubation and Ganoderma polysaccharides above 100 mg/L could partially antagonize this effect. Conclusion Ganoderma polysaccharides can partially antagonize PGE2-induced suppression ofmurine splenocyte IFN-γ and TNF-α mRNA expression.