肾细胞癌组织中CDK4、CyclinD1的表达及其意义

Expression of CDK4 and CyclinD1 in renal cell carcinoma and adjacent renal tissues

目的探讨肾细胞癌组织中CDK4、CyclinD1的表达水平及意义。方法应用实时荧光定量PCR法检测30例人肾细胞癌组织及癌旁肾组织中CDK4、CyclinD1的表达水平。结果CDK4和CyclinD1在肾癌细胞中的表达水平均明显高于癌旁肾组织,但CyclinD1的表达升高水平和比例高于CDK4表达的升高。CyclinD1/CDK4的比值,在肾癌组织中高于癌旁肾组织。结论CDK4和CyclinD1的过度表达可能引起细胞异常增殖、分化失控。CDK4和CyclinD1基因在肾细胞的发生、发展中可能起重要作用。

Objective To evaluate the significance of the expression CDK4 and CyclinD1 in renal carcinoma. Methods Real-time fluorescence quantitative PCR method was used to detect the expression level of CDK4 and CyclinD1 in 30 specimens of renal carcinoma tissue and 30 specimens of carcinoma-adjacent renal tissue. Results The expression level of CyclinD1 and CDK4 gene in renal carcinoma tissue was higher than that in carcinoma-adjacent renal tissues （P〈0. 05）, and the increasing magnitude of CyclinD1 was higher than that of CDK4. The ratio of CyclinD1 to CDK4 in renal carcinoma tissues was higher than that in carcinoma-adjacent renal tissues （P〈0.05）. Condusion The results suggest that over expression of CDK4 and CyclinD1 may result in abnormal renal cell reproduction and differentiation,leading to the generation of renal carcinoma.