摘要
目的探讨丝裂霉素C(MMC)对兔准分子激光上皮下角膜磨镶术(LASEK)后角膜雾状混浊(haze)的影响。方法对27只青紫兰兔双眼行LASEK手术,术后54眼随机分为3组,即0·02%MMC治疗组(18眼)、0·1%氟米龙治疗组(18眼)和空白对照组(18眼)。术后用裂隙灯显微镜观察角膜haze的形成情况;分别于1周、1月、2月取兔眼角膜,行光镜和透射电镜检查。结果术后1周、1月、2月,丝裂霉素C组角膜haze明显轻于对照组(P<0·05),MMC组术区前部基质内角膜成纤维细胞数较对照组和氟米龙组少(P<0·05),后两组间差异无显著性(P>0·05),三组间角膜上皮愈合时间差异无显著性(P>0·05)。结论LASEK术后MMC可抑制角膜基质成纤维细胞的增生及胶原纤维的合成,减少haze的形成,且无明显副作用,是一种有效抑制haze形成的药物。
Objective To investigate the effects of mitomycin C on haze after laser epithelial keratomileusis. Methods Twenty-seven pigmented rabbits underwent bilateral 193nm excimer laser epithelial keratomileusis to correct 10. 00 Diopters of myopia. All eyes were allocated randomly to treatment with 0. 02% mitomycin C during operation,or 0.,1% Fluorometholone after operation, or no medical treatment as control. Clinical and histopathologic examinations were made with slit-lamp microscope, light microscope and transmission electron microscope. Results In 1 week, 1 month, and 2 months after the operation, corneal haze was significantly less in mitomycin C group than that in the control group （ P 〈 0. 05 ）, and the number of fibroblas in the anterior stroma of ablation area was significantly smaller in mitomycin C group than that in the cortrol group or in Fluorometholone group （ p 〈 0. 05 ）. The difference between the Fluorometholone group and the control group was insignificant （ p 〉 0. 05 ）. The changes in time of reepithelialization were statistically insignificant among all groups （ P 〉 0. 05 ）. Conclusion Mitomycin C can reduce corneal haze after LASEK by inhibiting the proliferation of fibroblast and collagen and hasn＇t obvious toxicity on cornea. So it is an effective medicine for preventing the haze.
出处
《安徽医科大学学报》
CAS
北大核心
2006年第3期266-269,共4页
Acta Universitatis Medicinalis Anhui
基金
卫生部科研中标重点资助课题基金(编号:91309276)
