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大豆疫霉甘油醛-3-磷酸脱氢酶基因在病原物植物互作中诱导表达及其抗氧化作用在酵母遗传互补系统中的功能验证 被引量：2

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摘要 甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphatedehydrogenase,GAPDH)是真核生物细胞中一类功能丰富的蛋白质.采用抑制性差减杂交(suppressionsubtractivehybridization,SSH)的方法,筛选到一个在大豆疫霉侵染早期上调表达的、编码GAPDH的cDNA片段;克隆了该基因的全长序列,命名为PsGapdh.Southern杂交结果显示,PsGapdh在大豆疫霉基因组中含有3个拷贝.该基因编码的氨基酸序列具有GAPDH的保守结构域.在系统发育树中,PsGAPDH与两性绵霉(Achlyabisexualis)的GapC1同源性最高、与中华盒形藻(Odontellasinensis)和三角褐指藻(Phaeodactylumtricornutum)的GapC2同源性较高,三者聚类分布于GapC亚族的C-Ⅱ分支.RT-PCR分析表明,该基因在大豆疫霉侵染早期转录水平提高.该基因可以恢复酵母突变体?tdh1对H2O2的耐受性.上述结果提示,大豆疫霉的甘油醛-3-磷酸脱氢酶具有与酵母tdh1相似的抗氧化作用,且参与了大豆疫霉对寄主植物的侵染过程.
作者 曾娟 王源超 申贵 郑小波
机构地区 南京农业大学植物保护学院
出处 《科学通报》 EI CAS CSCD 北大核心 2006年第10期1175-1181,共7页 Chinese Science Bulletin
基金 国家重点基础研究发展计划(编号:2002CB111400) 国家自然科学基金(批准号:30471124) 教育部新世纪人才计划(批准号:NCET-04-0503)资助项目.
关键词 大豆疫霉 甘油醛-3-磷酸脱氢酶 病原物-植物互作 抗氧化
分类号 S435.651 [农业科学—农业昆虫与害虫防治]
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同被引文献35

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科学通报
2006年 第10期

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