Reversing multidrug resistance by RNA interference through the suppression of MDR1 gene in human hepatoma cells

AIM： To reverse the multidrug resistance （MDR） by RNA interference （RNAi）-mediated MDRI suppression in heparoma cells.METHODS： For reversing MDR by RNAi technology, two different short hairpin RNAs （shRNAs） were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into a highly adriarnycin-resistant HepG2 hepatorna cell line （HepG2/ADM）. The RNAi effect on MDR was evaluated by real-time PCR, cell cytotoxicity assay and rhodarnine 123 （Rh123） efflux assy. RESULTS： The stably-transfected clones showed various degrees of reversal of MDR phenotype. Surprisingly, the MDR phenotype was completely reversed in two transfected clones. CONCLUSION： MDR can be reversed by the shRNAmediated MDRI suppression in HepG2/ADM cells, which provides a valuable clue to make multidrug-resistant hepatoma cells sensitive to anti-cancer drugs.

瞄准：逆行多，由 RNA 干扰(RNAi ) 的药抵抗(MDR ) 调停了在肝细胞瘤房间的 MDR1 抑制。方法：为由 RNAi 技术颠倒 MDR，二不同短发卡 RNA (shRNAs ) 分别地被设计并且构造进 pGenSil-1 原生质标志。他们当时是进一根高度 adriamycin 抵抗的 HepG2 肝细胞瘤房间线(HepG2/ADM ) 的 transfected。MDR 上的 RNAi 效果被即时 PCR，房间细胞毒性试金和玫瑰精评估 123 (Rh123 ) 流出 assy。结果：stably-transfected 克隆显示出 MDR 显型的颠倒的各种各样的度。令人惊讶地， MDR 显型完全在二 transfected 克隆被颠倒。结论：MDR 能被调停 shRNA 的 MDRI 抑制在 HepG2/ADM 房间颠倒，它提供珍贵线索使 multidrug 抵抗的肝细胞瘤房间敏感到反癌症药。