Study on biological characters of SGC7901 gastric cancer cell-dendritic cell fusion vaccines

瞄准：检测 SGC7901 胃的癌症的生物人物房间树枝状的房间熔化疫苗。方法：推迟的生活 SGC7901 胃的癌症房间和树枝状的房间被导致是由聚乙二醇的 fusioned。纯熔化房间被选择文化与 HAT/HT 文化系统获得。熔化房间在文化的不同时间点被数，他们的生长曲线被拉反映他们的增生的活动。熔化房间在培养基也是有教养的调查他们是否能长成房间克隆。MTT 方法被用来在 T 淋巴细胞的增长上测试熔化房间的刺激能力。而且，熔化细胞被种进裸体老鼠观察他们是否能在这种免疫不全动物长成新种的肿瘤。结果：熔化房间比他们的父母房间有更弱的增生的活动和克隆能力。当他们是有教养的时，房间的计数没显著地增加，他们也不能在培养基长成房间克隆。在种了进裸体老鼠以后，熔化房间不能长成新种的肿瘤。T 淋巴细胞的增长上的熔化房间的刺激能力显著地比他们的父母树枝状的房间被增加。结论：SGC7901 胃的癌症房间树枝状的房间熔化疫苗比他们的父母房间有弱得多的增生的能力，但是他们使能力强壮激怒 T 淋巴细胞并且没有能力在免疫不全动物长成新种的肿瘤。这些是他们的反肿瘤简历治疗的生物基础。

AIM： To detect the biological characters of the SGC7901 gastric cancer cell-dendritic cell fusion vaccines.METHODS： The suspending living SGC7901 gastric cancer cells and dendritic cells were induced to be fusioned by polyethylene glycol. Pure fusion cells were obtained by selective culture with the HAT/HT culture systems. The fusion cells were counted at different time points of culture and their growth curves were drawn to reflect their proliferative activities. The fusion cells were also cultured in culture medium to investigate whether they could grow into cell clones. MTT method was used to test the stimulating abilities of the fusion cells on T lymphocytes＇ proliferations. Moreover, the fusion cells were planted into nude mice to observe whether they could grow into new planted tumors in this kind of immunodeficiency animals.RESULTS： The fusion cells had weaker proliferative activity and clone abilities than their parental cells. When they were cultured, the counts of cells did not increase remarkably, nor could they grow into cell clones in culture medium. The fusion cells could not grow into new planted tumors after planted into nude mice. The stimulating abilities of the fusion cells on T lymphocytes＇ proliferations were remarkably increased than their parental dendritic cells. CONCLUSION： The SGC7901 gastric cancer cell-dendritic cell fusion vaccines have much weaker proliferative abilities than their parental cells, but they keep strong abilities to irritate the T lymphocytes and have no abilities to grow into new planted tumors in immunodeficiency animals. These are the biological basis for their antitumor biotherapies.