骨肉瘤中survivin蛋白与PCNA的表达及其与预后关系的研究

The Expression of Survivin Protein and PCNA in Osteosarcoma and Its Relationship with Prognosis

[目的]研究骨肉瘤组织中survivin蛋白和PCNA的表达情况,及其与预后间关系。[方法]应用免疫组织化学SP法对46例原发性骨肉瘤组织中survivin蛋白与细胞增殖核抗原(PCNA)的表达水平进行检测。[结果]46例骨肉瘤组织中survivin蛋白表达阳性率为63.1%(29/46)。Survivin表达与外科Enneking分期有关,而与患者年龄、性别、肿瘤大小、部位、病理分型无关。Survivin蛋白表达与骨肉瘤细胞增殖活性显著性相关(P<0.01)。Survivin蛋白表达阳性者术后5年生存率(24.0%)显著低于表达阴性者(61.5%)(P<0.05)。[结论]Survivin蛋白在骨肉瘤中高表达,其表达与PCNA呈正相关;这种特异性表达对促进骨肉瘤细胞恶性增殖具有重要作用,survivin可成为评价骨肉瘤预后的分子标记。

[Purpose] To investigate the expression of survivin protein and proliferation cell nuclear antigen （PCNA） in human osteosarcoma and its relationship with the prognosis. [Methods] The expression of survivin and PCNA was detected by SP immunohistochemical technique in 46 cases with osteosarcoma. [Results] The positive rate of survivin was 63.1% in 46 cases with osteosarcoma. The expression of survivin was correlated with Enneking staging, but not correlated with the age, gender, tumor size, primary location and histological grade. The survivin expression was positively related with activity of PCNA in osteosarcoma （P〈0.01）. The 5-year survival rate in patients with survivin positive expression was 24.0% which was much lower than that in patients with survivin negative expression （61.5%）（P〈0.05）. [Conclusion] Over expression of survivin in osteosarcoma was positive relation with PCNA, which may play an important role in maglignant proliferation of oxteosarcoma cells. Survivin expression might be a useful prognostic marker for osteosarcoma.