内分泌腺来源的血管内皮生长因子在卵巢上皮性肿瘤中的表达及意义

Experssion and significance of EG-VEGF mRNA in ovarian epithelial tumors

目的研究内分泌腺来源的血管内皮生长因子(EG-VEGF)在卵巢上皮性肿瘤的表达,以明确其与卵巢癌发生、发展的关系。方法采用RT-PCR法检测卵巢上皮性肿瘤(恶性、临界恶性、良性)及正常卵巢组织中EG-VEGFmRNA的表达情况。结果EG-VEGFmRNA在卵巢上皮性癌的表达率及相对含量均高于卵巢临界恶性上皮肿瘤及良性上皮肿瘤,差异有显著性意义(P<0.05),而后二者EG-VEGFmRNA的表达无明显差异(P>0.05)。早期卵巢癌EG-VEGFmRNA的相对含量明显高于晚期癌(P<0.05)。正常卵巢组织中,EG-VEGFmRNA的表达率高于卵巢临界恶性及良性上皮肿瘤(P<0.01),EG-VEGFmRNA的相对含量亦高于三种卵巢上皮性肿瘤(P<0.01)。结论EG-VEGF虽非主要来源于上皮细胞,但它与卵巢癌的发生有关,可能是衡量卵巢肿瘤良恶性的指标。该基因表达可能是卵巢癌发生过程中相对早期的事件,抗EG-VEGF靶向基因疗法有望对早期卵巢癌发挥治疗作用。

[Objective] We detected the expression of EG-VEGF mRNA in ovarian epithelial tumors to explore the relationship between the factor and the growth and development of ovarian epithelial carcinoma（OEC）. [Methoils] We detected the expression of EG-VEGF mRNA in ovarian epithelial tumors （malignant, borderline, benign） and normal ovarian tissues by RT-PCR technique. [Results] Both the expression rate and the relative content of EG-VEGF mRNA in OEC were signifieantly different from ovarian borderline epithelial tumors and benign epithelial tumors （P 〈0.05）, but no significant difference was identified between ovarian borderline epithelial tumors and benign ones （P 〉0.05）. The relative content of EG-VEGF mRNA in early stage OEC was higher than that in late stage OEC significantly （P 〈0.05）. In normal ovarian tissues, the expression rate of EG-VEGF mRNA was higher than that in ovarian borderline and benign epithelial tumors significantly（P 〈0.01）. For the relative content, was higher than that in ovarian epithelial tumors significantly （P 〈0.01）. [Conclusions] EG-VEGF is not derived from epithelial cells mainly, but it has relationship with the occurrence of ovarian malignant tumors, and it maybe an index reflecting the benignancy or malignancy of ovarian tumors. The expression of EG-VEGF gene is an early event in the course of ovarian eareinoma＇s occurrence, maybe EG-VEGF-targeted therapy is useful for the therapy of early stage ovarian carcinoma.