复发性阿弗他溃疡与病毒感染的相关性研究

Study on correlation between recurrent aphthous ulcer and virus infection

目的探讨复发性阿弗他溃疡(RAU)与人巨细胞病毒(HCMV)和单纯疱疹病毒(HSV)感染的相关性。方法采用酶联免疫分析法(ELISA)和聚合酶链反应技术(PCR)联合检测176例RAU患者和120例健康对照者HCMV和HSV血清IgM抗体及溃疡组织或脱落上皮细胞中的病毒DNA,并进行综合分析。结果RAU患者组病毒阳性率(71.02%),明显高于健康对照组(7.50%,P<0.05)。其中单项HCMV的阳性率为47.16%,HSV的阳性率为52.27%,二者复合感染的阳性率为28.41%,三者与对照组相比,均具有显著性差异(P<0.05)。但HCMV与HSV二者之间的阳性率差异无统计学意义(P>0.05)。结论HCMV和HSV感染与RAU的发病密切相关,是其病因中的重要致病因素。RAU患者不只是一种病毒感染,而且存在着不同病毒感染状态,病毒的复合感染也占有一定比例;联合检测HCMV和HSV的IgM抗体与病毒DNA,可显著提高病毒感染的阳性检出率,有利于RAU的病因诊断和治疗。

[Objective] To explore the correlation between recurrent aphthous ulcer （RAU） and infection of human cytomegalovirus （HCMV）, herpes simplex virus （HSV）. [Methods] Techniques of Enzyme-Linked Immunoadsorbent Assay （ELISA） and polymerase chain reaction （PCR） were combined to detect the levels of serum IgM Antibody of HCMV,HSV and the virus DNA in ulcer tissues, dropped epidermis cells from 176 patients with RAU and 120 persons acted as health control. Data were comprehensively analyzed. [Results] The ratio of virus positive in RAU patient group was 71.02%, which was higher than that in control group （7.50%） significantly, the positive rate of single HCMV infection was 47.16%, the positive rate of the single HSV infection was 52.27%, the positive rate of the combined infection was 28.41%, there were significant difference between the virus infection and control group （P 〈0.05）. But there was no statistical significance between the HCMV positive rate and the HSV positive rate （P 〉 0.05）. [Conclusion] Infection of HCMV, HSV was closely related to RAU. RAU, combined with virus infection, is not a simple virus infection, there are different virus infections, combined-infection also takes certain proportion. The combined-detection of serum IgM Antibody of HCMV, HSV and the virus DNA can increase the detection rate of virus infection significantly, it is benefit to diagnosis and therapy of RAU.