环境条件对极大螺旋藻金属元素吸收的影响

EFFECTS OF DIFFERENT ENVIRONMENTAL CONDITIONS ON THE ABSORPTION OF METAL ELEMENTS IN SPIRULINA MAXIMA

研究了不同微量元素浓度和不同EDTA加入量对极大螺旋藻金属元素吸收的影响。结果显示,增加培养基中的微量元素含量有利于藻细胞对金属元素的吸收。少量EDTA的加入,有助于部分金属元素的吸收,较大量的EDTA可阻碍金属元素的吸收。藻细胞的吸附实验表明,极大螺旋藻对Co、Ni、Cu、Zn、Pb有很强的吸附能力,死细胞与活细胞的吸附能力相近,表明这一吸附过程不依赖于生理活动。

The effects of different concentrations of trace element solutions (A5 and B6) and different quantites of EDTA in media on the contents of metal elements in Spirulina maxima were studied. The results show that by increasing the concentrations of A5 and B6 trace elements from 1mL/L (Zarrouk medium) to 3mL/L in medium, the contents of most metal ions in algae increased. However, if A5 and B6 were increased to 5mL/L, no more increase of metal contents was observed except for K, Zn and Pb. The metal contents of algae were decreased when increasing EDTA in medium, while the contents of Cr, Fe, Sr and Ti in algae were, when EDTA was 0. 08g/L in medium, higher than those in the algae cultured without EDTA. The results suggest that higher concentrations of metal ions in medium increased algae absorbing metal ions and EDTA can make algae absorbing metal ions decreased.The experiment on the adsorption of heavy metal elements on intact algal cells shows that 5. maxima strongly adsorbed Co, Ni, Cu, Zn and Pb ions in 30 minutes. The adsorption capacity of cells killed by formol was comparable to living cells, strongly suggesting that the adsorption was independent of physiological reactions.