多药耐药相关蛋白在乳腺癌组织中的表达及预后关系

The Prognostic Significance of Multidrug Resistance-associated Protein 1(MRP_1) Expression in Primary Breast Carcinoma

目的研究多药耐药相关蛋白(Multidrug resistance-associated protein 1,MRP1)在乳腺癌组织中的表达,评估其在乳腺癌预后中的作用。方法采用免疫组织化学方法(lmmunohistochemistry,IHC)检测60例手术切除的乳腺癌组织中MRP1的表达,并分析其与临床、病理特征的关系及对预后的影响。结果(1)MRP1在乳腺癌组织中的阳性表达率为85.0%(51/60例),其中高表达者32例(53.3%);(2)腋淋巴结转移阳性者MRP1表达水平明显高于腋淋巴结转移阴性者(P<0.05),MRP1表达与月经状况、肿瘤大小、组织分级和激素受体状况均无关(P>0.05);(3)Kaplan-Meier生存分析结果表明:MRP1表达与无病生存期明显相关(P<0.05),但和总生存期无关(P>0.05);(4)COX多因素分析显示肿瘤大小、腋淋巴结转移和雌激素受体状况仍是影响无病生存期和总生存期的重要因素(P<0.05),同时MRP1表达与和无病生存期和总生存期均无关(P>0.05)。结论MRP1在乳腺癌组织中具有较高的表达水平,但与乳腺癌患者预后无关。

Objective Intrinsic and/or acquired resistance is the major obstacle to overcome in the treament of patients with breast cancer. The aim of the present study was to investigate the relationship between the expression of muhidrug resistance -associated protein 1 （ MRP1） with the clinicpathological characteristics and the prognosis in primary breast cancer patients. Metgods Expression of MRP1 in 60 breast cancer patients was determined by immunohistochemistry on fomudin - fixed, paraffin - embedded tumor section, Remits （ 1） MRP1 expression was observed in 51 of 60（85.0% ）. （2）MRP1 expression was more frequently observed in patients with lymph nodemetastasis. No relation can be established between expression of MRP1 and other elinieophathological parameters. （3） Kaplan -Meier analyses revealed that MRP1 expressiononly associated with disease - free survival （DFS） and didn＇ t with overall survival （ OS ）. （4） In multivariate Cox regression analyses, MRP1 expression didn＇t associate with DFS, and OS. In addition, tumor size, lymph node metastasis and ER were independent prognostic thctors for DFS and OS（ P 〈 0.05）. Conclusion Our results suggested that MRP1 is frequently expression in primary breast cancer, but MRPt is not a poor prognostic factor in patients with breast cancer.