斑叶竹节海棠的离体快速繁殖研究

Study on In vitro Propagation of Begonia macuiata Radd.

本文报道了斑叶竹节海棠的离体快速繁殖实验及结果．以斑叶竹节海棠的叶片为外植体，在含有不同浓度配比的６－ＢＡ和ＮＡＡ的ＭＳ培养基上诱导生芽，其中以０．４ｍｇ／Ｌ６－ＢＡ和０．２ｍｇ／ＬＮＡＡ的配比为最佳，生芽率达９４％．以此培养基为增殖培养基成苗．当苗长至３－４ｃｍ时．切下苗在１／２ＭＳ或１／２ＭＳ＋０．１ｍｇ／ＬＩＡＡ的培养基上生根，获得了再生植株并移栽成活，成活率达９０％．在增殖和生根过程中，用食糖代替蔗糖．用自来水代替蒸馏水亦取得了较好的效果，为斑叶竹节海棠的批量化生产奠定了基础．

The buds were induced when leaf segmentS of B. macuiata Radd. were cultured on MS media supplemented with different combinations of 6 - BA and NAA. The best combination was 0. 4 mg/L 6 - BA and 0. 2 mg/L NAA,in wich the buding percentage and the buds grew into shoots. When shoots reached 3 - 4 cm, they were cut off and cultured in 1/2MS medium or supplemented with 0. 1 mg/L IAA, and roots were produced. Plants were translated into pots, the survival percentage was about 90 %. During Propagation and rooting, chemical pure sucrose can be replaced by white sugar, and distilled water by tap water. This is more economical in commercial production of B .macuiata Radd..