若干因子对鸡冠花悬浮培养中花色素苷积累的影响

Effects of Several Biological Factors on Anthocyanin Accumulation of Celosia cristata var.pyramidalis in Cell Suspension Culture

本文研究几种植物生长调节剂和蔗糖浓度对鸡冠花细胞悬浮培养中花色素苷积累的影响。结果表明,细胞分裂素KT使花色素苷积累明显高于6-BA,且KT在2μmol/L时积累量最高;2,4-D在2μmol/L时对花色素苷积累效果明显,其它浓度的2,4-D和NAA对花色素苷积累效果不明显。高浓度蔗糖有利于花色素苷积累;MS+2,4-D(2μmol/L)+KT(2μmol/L)+蔗糖(292mmol/L)为鸡冠花悬浮细胞培养生产花色素苷的最佳培养基。研究中还发现,在黑暗条件培养下无花色素苷积累,推断光是诱导花色素苷积累的主要因素。随着继代次数的增加,花色素苷含量明显增高,但到第4代时基本稳定。

The effects of different concentrations of growth regulators and sucrose on anthocyaninproduction of Celosia cristata vat. pyramidalis in cell suspension culture were studied. Anthocyaninaccumulation treated with KT was significantly greater than that treated with 6-BA, and the maximalproduction of anthocyanin was obtained when treated with KT at 2 μ mol/L. 2,4-D 2 μmol/L and high concentration of sucrose could promote the accumulation of anthocyanin. MS ＋ 2,4-D 2 μmol/L ＋ KT 2 μ mol/L ＋ sucrose 292mmol/L gave the best result for anthocyanin accumulation.Light was essential for the induction of anthocyanin. The content of anthocyanin increased with themultiplication of suspension cells.