摘要
综述了聚合酶链式反应(PCR技术)的原理、方法及其在各个领域的广泛应用。PCR技术以其敏感性、特异性、简便、快速的优点,现已广泛应用于微生物检测,尤其对培养困难的细菌检测和抗原结构复杂的细菌鉴定方面,具有常规方法无法比拟的优越性。本文主要介绍了PCR技术检测沙门氏菌,核细胞增生李斯特菌,金黄色葡萄球菌和致病性大肠杆菌这4种食品中的主要致病菌。
This article summarized the principle, method and application in many fields of PCR technology. PCR has the sensitive, differential, simple, convenient and fast virtues, which have been used in microorganism inspection , especially in bacteria inspection incubated difficultly and with complex structure antigen. Salmonella, Listerimonocytogenes, Staphylococcusaureus, Escherichia coli the four important pathogens were intruoduced in the article.
出处
《食品研究与开发》
CAS
北大核心
2006年第5期107-109,99,共4页
Food Research and Development
