应用马血、兔血、羊血巧克力琼脂培养基分离流感嗜血杆菌的效果评价

Effect evaluation of chocolate agar media made of horse,rabbit and sheep blood using for isolate haemophilus influenzae

目的比较马血、兔血、羊血巧克力琼脂培养基痰培养流感嗜血杆菌的检出率和生长情况.方法将送检的痰标本及流感嗜血杆菌标准菌株ATCC10211分别接种于马血、兔血、羊血巧克力琼脂培养基上,置烛缸中,36℃培养24 h,比较流感嗜血杆菌检出率及其生长情况.结果 （1）马血、兔血、羊血巧克力琼脂培养基痰培养流感嗜血杆菌的检出率分别为34.3%、35%、8.3%.（2）标准菌株ATCC10211在马血、兔血巧克力琼脂培养基上生长良好,菌落直径0.5～1.0 mm,而在羊血巧克力琼脂培养基上生长差,菌落大小如细粉末状,肉眼难见.结论痰培养流感嗜血杆菌使用马血、兔血巧克力琼脂培养基效果优于羊血巧克力琼脂培养基.

Objective To compare the isolate rates of haemophilus influenzae in sputum by chocolate agar media made of home, rabbit and sheep blood, and the growth of haemophilus influenzae on the three kinds of choco- late agar media. Methods Sputums and Haemophilus influenzae ATCC10211 were streaked on the three kinds of chocolate agar media and incubated at 36℃ in a candle jar for 24 hours. The isolate rates of haemophilus influenzae by three kinds of chocolate agar media and the growth of haemophilus influenzae ATCC10211 were compared. Resuits The isolate rates of Haemophilus influenzae in sputum by chocolate agar media made of horse, rabbit and sheep blood were 34.3 %, 35 %, 8.3 % respectively. Haemophilus influenzae ATCC10211 grew very well on chocolate agar media made of horse and rabbit blood, its diameter of colony was 0.5 - 1.0mm. But it did not grow well on the chocolate agar media made of sheep blood ,its colony was as small as a sand and hard to be seen with naked eye. Conclusion Chocolate agar media made of horse and rabbit blood are better than the chocolate agar media made of sheep blood in haemophilus influenzae isolation in sputum.