抗肿瘤活性物SYUIQ-5对大鼠肝组织细胞色素P450基因表达的影响

Effects of SYUIQ-5,a novel antitumor agent,on gene expression of hepatic cytochrome P-450 in rats

目的研究抗肿瘤活性物SYUIQ-5对大鼠肝组织细胞色素P(CYP)450基因表达的影响。方法抗肿瘤活性物SYUIQ-5,0.1mg/kg、5mg/kg、10mg/kg三个剂量,雄性SD大鼠经腹腔注射连续处理5d后,取肝组织,提取总RNA,应用反转录-聚合酶链反应(RT-PCR)检测CYP3A1、2B1/2、1A1、1A2和2E1信使核糖核酸(mRNA)的表达。结果与空白对照组相比,抗肿瘤活性物SYUIQ-5可显著诱导CYP1A1mRNA的表达,对CYP3A1、2B1/2、1A2和2E1mRNA表达无影响。结论抗肿瘤活性物SYUIQ-5可显著诱导大鼠肝组织CYP1A1基因表达,对CYP3A1、2B1/2、1A2和2E1的基因表达无影响。

Objective To study the effect of SYUIQ-5, a novel antitumor agent,on gene expression of hepatic cytoehrome P450 in rats. Methods Male Sprague-Dawley rats were administered daily intraperitoneal dose of SYUIQ-5（0. lmg/kg,5mg/kg and 10mg/kg body weight） for 5 consecutive days. The levels of CYP 3A1,2B1/2, 1A1,1A2 and 2E1 mRNA in rat liver were analyzed by reverse transeription-polymerase chain reaetion（RT-PCR）. Results The level of gene expression of CYP 1A1 was increased significantly compared with the control. The levels of CYP 3A1,2B1/2,1A2 and 2E1 mRNA were not changed by SYUIQ-5 treatment compared with the control. Conclusion A significant increase in the CYP 1A1 rather than CYP3A1,2B1/2,1A2 and 2E1 gene expression by SYUIQ-5 treatment is observed.