沙棘叶子提取物对白化病白鼠由铬诱导氧化胁迫的抗氧化活性评估

Evaluation of antioxidant activity of leaf extract of Seabuckthorn(Hippophae rhamnoides L.) on chromium(VI) induced oxidative stress in albino rats

本研究报道沙棘(胡颓子科)对雄性白化病白鼠由铬诱导氧化胁迫的抗氧化活性。氧化胁迫通过对小白鼠进行强制性喂养30 d,以浓度为30 m g/kg的铬相对体重比例的重铬酸钾盐实现。铬促使体重下降,而却明显增加了器官与体重比例。铬处理明显减少了谷胱甘肽的降低,增加了丙二醛和肌氨酸磷酸激酶的水平;而且它还加强了血清中谷氨酸草酰乙酸转移酶和谷氨酸丙酮酸转移酶的浓度。用不同剂量的沙棘叶子提取物(乙醇提取)对保护铬元素诱导的氧化胁迫进行了评估,结果表明叶子提取物在浓度为100到250 m g/kg铬与体重比的情况下可以明显保护动物避免由铬所诱导的氧化伤害。

The present study reports the antioxidant activity of Seabuckthorn （Hippophae rhamnoides）, family Elaegnaceae, on chromium induced oxidative stress in male albino rat＇s. Oxidative stress was induced in the rats by force-feeding of potassium dichromate equivalent to a dose of 30 mg/kg body weight （BW） of chromium （VI） for 30 days. Administration of chromium decreased the body weight and increased organ to body weight ratio significantly. Chromium treatment significantly decreased reduced glutathione （GSH）, and increased malondialdehyde （MDA） and creatine phosphokinase （CPK） levels; further it also enhanced glutamate oxaloacetate transferase （GOT） and glutamate pyruvate transferase （GPT） levels in the serum. Different doses of the alcoholic leaf extract of Seabuckthorn were evaluated for the protection against the chromium induced oxidative stress. The results show that the leaf extract at a concentration of 100 and 250 mg/kg BW protected the animals from the chromium induced oxidative injury significantly.