砂地柏提取物、鬼臼毒素和脱氧鬼臼毒素对粘虫幼虫生长发育和食物利用的影响

Effects of extracts from Sabina vulgaris Ant., podophyllotoxin and deoxypodophyllotoxin on growth and food utilization of Mythimna separata larvae

采用重量法研究了砂地柏Sabina vulgaris Ant.果实乙醇提取物、鬼臼毒素和脱氧鬼臼毒素等3种植物源拒食性物质在AFC30、AFC50及AFC80剂量处理下对粘虫Mythimna separata幼虫生长发育和食物利用的影响.结果表明,3种供试物质对粘虫幼虫正常的生长发育历期具有明显的延缓作用,可显著延长试虫龄期.2种鬼臼类物质在处理期内均可造成粘虫幼虫相对生长率和相对代谢率的明显降低,并且表现出与剂量的相关性,但停止处理3～5天后,又都能恢复到正常水平;3种物质对粘虫幼虫食物近似消化率没有明显影响.处理期内食物转化率和利用率显著增加,停止处理后,均可恢复到正常水平;不同物质处理间存在一定差异,同一物质不同处理剂量间差异不明显.

We assessed the effects of the extracts from Sabina vulgaris Ant., and two podophyllotoxin analogues, i. e., podophyllotoxin and deoxypodophyllotoxin, which are main insecticidal secondary components of S. vulgaris, on growth and food utilization of Mythimna separata larvae with weight method under treatment with three doses of AFC30, AFC50 and AFC80. The results showed that the tested chemicals had obvious inhibiting activity on growth and development of M. separata larvae, and the larval stage was significantly longer when controlled with the three chemicals under above doses. During treated periods, the related growth rate （RGR） and related metabolizing rate （RMR） were significantly lower when controlled with the two podophyllotoxin analogues, and the higher the dose, the lower the rates. But in 3 - 5 days post treatment, these two rates recovered to common levels. The three chemicals had no obvious effects on larval approximate digestibility （AD）. During the controlled period, the efficiency of conversion of digested food （ECD） and efficiency of conversion of ingested food （ECI） significantly increased. In 3 - 5 days post treatment, these two rates also recovered to common levels. There was a certain extent difference of ECI and ECD among controlled chemicals, but no obvious difference among doses.