一种适合于成熟脐橙果皮和果肉的RNA提取方法(英文)

Efficient Isolation of RNA from Fruit Peel and Pulp of Ripening Navel Orange(Citrus sinensis Osbeck)~*

研究了一种适合脐橙果实成熟过程中有效的RNA提取方法。结果表明利用该方法从不同成熟时期果实的果皮和果肉中提取的RNA可以有效用于RT-PC,cDNA-AFLP和RNA杂交。其A260/A230的比值超过2.0,A260/A280的比值在1.65-1.92的范围之间。另外该方法也证明可以广泛用于柑橘叶片,未成熟的幼果,枳壳幼苗和柑橘愈伤组织的RNA提取,是一种安全、方便和适用性较广的RNA提取方法。

An efficient RNA isolation method was established in the present paper. RNA extracted from peel and pulp collected at different ripening time was successfully used for reverse transcription-PCR （RTPCR） without DNase treatment, complementary DNA amplified fragment length polymorphism （cDNA-AFLP）, and RNA blotting. The A260/A230 ratios were higher than 2. 0, and the A260/A280 ratios ranged from 1.65 to 1.92. In addition, the protocol is safe, convenient and proved to be widely applicable, since it was successfully employed for RNA extraction from citrus leaves, immature fruit, Poncirus seedling and citrus callus as well.