刺五加皂苷脂质体的制备及对肝癌移植瘤的抑制作用

Preparation of Acanthophopanaxenticosus Liposomes and Its Therapeutic Effect on Implanted Hepatocellular Carcinoma in Mice

目的:制备刺五加皂苷脂质体并研究其对肝癌移植瘤的抑制作用。方法:采用薄膜分散-超声法制备刺五加皂苷脂质体并检测其理化性质,用流式细胞仪检测此载药脂质体对HepG2肝癌细胞周期和凋亡的影响,并观察刺五加皂苷脂质体荷瘤小鼠的抑瘤作用。结果:所制备的脂质体封率为63.43%,刺五加皂苷脂质体组与游离刺五加皂苷组均使肝癌细胞G0/G1期和S期比例明显下降,并观察到细胞凋亡,高剂量刺五加皂苷脂质体组荷瘤小鼠的抑瘤率(62.21%)显著高于低剂量组(45.23%)和游离刺五加皂苷组(46.16%,P均<0.05),而低剂量刺五加皂苷脂质体组与游离刺五加皂苷组无明显差异(P>0.05)。结论:刺五加皂苷脂质体能抑制肝癌细胞Hep G2增殖和诱导细胞凋亡,对小鼠肝癌移植瘤的生长有明显抑制作用。

Objective： To prepare Acanthophopanaxenticosus（ASS） liposomes and investigate its therapeutic effect on implanted hepatocellular carcinoma. Methods ：The Acanthophopanaxenticosus lipesomes were prepared by the thin- film hydration and ultrasonic technique. The flow cytometry were applied to study cell cycle and apoptosis of the hepatocellular cancer cell line Hep G2 and the tumor growth inhibition rate in mice were calculated. Results ： The ASS liposomes encapsulation percentage is 63.43%. The cell number rate in C0/C1 phase and S phase were reduced signifieandy. The inhibitory effect of high- dose ASS liposomes group（62. 21% ） was stronger than that of low- dose ASS liposomes group（45.23% ）or single ASS group（46. 16%, P 〈 0.05） ;Compared with the single ASS group,the inhibition rate in the low -dose ASS liposomes group was no significandy difference. Conclusion： The ASS liposomes can inhibit signifieandy proliferation of hepatocellular on tumor growth of cancer cell Hep C2 and induce apoptosis ,can also inhibit tumor growth in mice.