荆芥挥发油对白三烯拮抗活性的实验研究

Effect of Jingjie Volatile Oil on Leukotrienes levelsin Rats

目的:观察荆芥挥发油(Sto)对白三烯类炎症介质的影响,探讨其抗炎作用机理。方法:角叉菜胶制备大鼠急性胸膜炎炎症模型,荆芥挥发油连续预防给药7天,ELISA法测定模型大鼠血清中LTB4含量;大鼠连续灌胃给予荆芥挥发油7天,制备腹腔白细胞悬液,加入外源性花生四烯酸,RP-HPLC法测定LTB4和LTC4的生成含量。结果:荆芥挥发油0.2、0.1mL/kg能明显降低模型大鼠血清LTB4含量,0.2mL/kg能显著减少花生四烯酸代谢产物LTB4和LTC4的生成。结论:荆芥挥发油具有拮抗白三烯活性的作用,是其良好抗炎作用的机制之一。

Objective： To study the effect of Jingjie Volatile Oil （Sto） on leukotrienes levels in vivo and ex vivo as well as the mechanism of anti - inflammatory action of Sto. Methods ： The rats were administrated with Sto for 7 days. The LTB4 of serum in carrageenan - induced acute inflammation in rats were measured by ELISA, and the levels of LTB4 and LTC4 in rat leucocyte were determined with RP- HPLC ex vivo. Results： Sto（ 0.2,0.1mL/kg ） significantly decreased the concentration of leukotrieneB4 （ LTB4 ） in serum in carrageenan -induced acute inflammation in rats, Sto（ 0.2mL/kg ） decreased the metabolites concentration of arachldonle acid in leucocyte sample resulted from abdominal cavity in rats that pre - treated with Sto. Conclusion ： It suggests that the anti -inflammatory effects of Sto, due to its suppression effect on metabolism of arachldonie acid（A.A）, may allow it to alleviate the inflammation symptoms.