摘要
研究成年家兔腓肠肌内外侧头和胫前肌的肌纤维构成比例与分布.应用烟酰胺腺嘌呤二核苷酸四唑氧化还原酶（NADH-TR）染色法,观察比较各型肌纤维的组织化学特性.结果表明：NADH-TR染色,依据其肌纤维内线粒体和肌质网显示紫色或紫蓝色,肌原纤维中的肌动蛋白和肌球蛋白不着色,可以分为Ⅰ型、ⅡA型、ⅡB型肌纤维,横切面呈多边形或椭圆形,以镶嵌交叉方式排列.三种类型的肌纤维在腓肠肌内侧头中分别约占15.2%,27.4%和57.4%,在站立负荷中提供足够肌力和稳定机体姿势的作用;在腓肠肌外侧头中分别约占13.7%,65.4%和20.9%,主要参与力量和速度运动,提供奔跑,跳跃中的推进动力;在胫前肌中约占10.9%,52.3%和36.8%,维持跗关节稳定,参与保持足弓形状与弹性,完成足跖屈和足内翻运动.腓肠肌内外侧头和胫前肌的三型肌纤维直径有所差异,其中Ⅰ型纤维为57.50～58.40 μm,ⅡA型纤维为59.25～75.40 μm,ⅡB型纤维为73.25～97.85 μm.ⅡB型纤维的横切面积最大,ⅡA型纤维的横切面积中等,Ⅰ型纤维的横切面积最小.
In order to observe the proportion and the distribution of fibe types in the gastrocnemius muscles and tibialis anterior muscles, histochemical characteristics of the muscles fibers from adult rabbits were observed by nicotinamide adenine dinuclectide tetrazolium oxidoreductase （NADH-TR） staining. The results revealed that these muscle fibers were divided into type Ⅰ,ⅡA and IIB fiber,and showed a mosaic pattern of fiber types distribution. The percentage of type Ⅰ,ⅡAA and ⅡB fibers were separately 15.2% , 27.4% and 57.4% of the total amount of medial gastrocnemius muscles, and about 13.7%, 65.4%and 20.9% in lateral gastrocnemius muscles. We suggeste that the medial gastrocnemius produce relative high tension in maintaining the standing posture and stabilizing organism, and the lateral gastrocnemius serve more in velocity and force. In the tibialis anterior muscles, the percentage of Ⅰ,ⅡA and ⅡB fiber types are about 10.9% , 52. 3% and 36.8%. It is designed mainly to stabilize the ankle and to help to maintain the arches of foot, and take part in plantarflexing the ankle. The diameter of type Ⅰ fiber was among 57. 50-58.40μm, type IIA fiber was among 59.25-75.40μm, type IIB fiber was among 73. 25-97. 85μm in medial gastrocnemius, lateral gastrocnemius and tibialis anterior muscle. Transverse section of type liB fiber was the biggest, transverse section of type ⅡA fiber was medium, and transverse section of type Ⅰ fiber was the smallest.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第6期625-628,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
江苏省教育厅与校自然科学基金资助项目
