摘要
A method of High-performance liquid chromatography(HPLC) coupled with photodiode-array detection(DAD)techniques was developed for the simultaneous determination of chlorogenic acid,caffeic acid,echinacoside and cichoric acid in the roots,stems,leaves,and flowers respectively from Echinacea samples(Echinacea pallida,E.angustifolia,and E.purpurea).The analyses were carried out on a Shiseido C18 column,with mobile phase gradient.Detection wavelength was set at 329nm.The recovery,precision,stability and linearity for different compounds were satisfactory.
A method of High - performance liquid chromatography (HPLC) coupled with photodiode - array detection (DAD) techniques was developed for the simultaneous determination of chlorogenic acid, caffeic acid, echinacoside and cichoric acid in the roots, stems, leaves, and flowers respectively from Echinacea samples (Echinacea pallida, E. angustifolia, and E.purpurea) . The analyses were carried out on a Shiseido C18 column, with mobile phase gradient. Detection wavelength was set at 329nm. The recovery, precision, stability and linearity for different compounds were satisfactory.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2006年第6期749-752,共4页
Chemical Research and Application