我国分离人H5N1禽流感病毒血凝素基因特性的研究

Analysis of human H5N1 virus hemagglutinin gene isolated from the mainland of China

目的分析我国分离的人高致病性禽流感H5N1病毒的抗原性以及基因特性。方法对所分离的H5N1病毒的血凝素基因和神经氨酸酶基因进行序列测定。结果对血凝素基因的序列测定结果表明从我国分离的H5N1病毒具有较高的同源性,但是与越南、泰国等国家分离的H5N1病毒明显不同。序列测定的结果同时表明无论是受体特异性还是连接肽都是禽源的。结论目前我国分离的H5N1病毒属于同一个组,与泰国、越南分离毒株不同,并且发现病毒的特性仍然是禽源的,没有发现从禽到人的重组或者重配。

Objective To analyze the genetic and antigenic characteristics of human HSN1 virus isolated from the mainland of China. Methods The hemagglutinin （HA） gene of human H5N1 virus were sequenced and analyzed. Results The results of HA gene sequencing showed that all the virus isolates belong to the same group because of the high similarity, but they were different with the virus isolated from Thailand and Vietnam. The sequence data also showed that the receptor specificity and the connecting peptide between HA1 and HA2 are still avian influenza origin. Conclusion The virus isolates from mainland of China until now belong to the same group and are different from the virus isolated from Thailand and Vietnam, and there is no evidence showing the humanavian influenza reassortant and recombination.