HPLC法测定苦参及苦参汤中黄腐醇和苦参啶的含量

HPLC determination of xanthohumol and kuraridin in Sophora flavescens Ait.and the effective fraction of Kushen decoction

建立测定苦参及苦参汤有效部位中黄腐醇和苦参啶含量的高效液相色谱方法。方法:Phenomenex Luna C_(18)(4.6mm×250mm,5μm)色谱柱,流动相为甲醇-0.38%磷酸水溶液(75:25),流速为1.0mL·min^(-1),检测波长为365nm。结果:黄腐醇和苦参啶分别在2.528×10^(-3)～1.264×10^(-2)μg和0.204～1.020μg范围内线性良好,相关系数 r 分别为0.9997和0.9998。3组样品黄腐醇平均回收率为100.9%,99.77%,99.26%,RSD(n=3)为1.6%,I.1%,2.0%;苦参啶平均回收率为97.66%,97.84%,96.61%,RSD(n=3)为1.4%,2.1%,1.5%。结论:本方法操作简便,分离效果好,灵敏度高,重复性好,可用于控制苦参及苦参汤有效部位质量。

Objective： To establish a method for the determination of the contents of xanthohumol and kuraridin in Sophora flavescens Ait. and the effective fraction of Kushen decoction by HPLC. Methods： The determination was carried out with Phenomenex Luna C18 column（4.6 mm× 250 mm,5 μm）,using methanol-0. 38% phosphoric acid（75： 25）as the mobile phase at a flow rate of 1 mL·min^-1 and detected at the wavelength 365 nm. Results： The calibration curves of xanthohumol and kuraridin were in good linearity over the range of 2. 528 ×10^-3 - 1. 264 ×10^-2 μg and 0. 204 - 1. 020 μg,with r =0. 9997 and 0. 9998 ,respectively. The average recoveries for xanthohumol and kuraridin of three batches of samples were 100. 9% ,99.77% ,99. 26% and 97.66% ,97.84% ,96.61% , with RSD （ n = 3 ） were 1.6%, 1.1%, 2. 0% and 1.4%, 2. 1%, 1.5% respectively. Conclusion： The method is simple, sensitive, accurate and reproducible, and can be used to control the qualities of Sophora flavescens Ait. and the effective fraction of Kushen decoction.