高效液相色谱法测定酸枣仁中白桦脂酸的含量

HPLC determination of betulinic acid in Semen Ziziphi Spinosae

目的:建立高效液相色谱测定酸枣仁中白桦脂酸含量的方法。方法:采用反相高效液相色谱法,以 ALCHROM^(TM) HyperSil C_(18)(250mm×4.6mm,5μm)为色谱柱,甲醇-水-三乙胺-冰醋酸(85:15:0.05:0.03)为流动相,检测波长210nm,流速0.8mL·min^(-1),柱温25℃。结果:白桦脂酸的线性范围为0.8～4.0μg,r=0.9995,平均回收率为102.5%,RSD=2.0%。结论:该方法准确、快速、灵敏、重现性好,可用于酸枣仁中白桦脂酸的定量分析。

Objective：To establish an HPLC method for the determination of betulinic acid in Semen Ziziphi Spinosae. Method：The separation was achieved on a ALCHROMTM Hyper Sil C18 column （250 mm ×4. 6 mm,5 μm） at 25 ℃. The mobile phase composition was methanol - water - triethylamine - glacial acetic acid （ 85： 15： 0. 05： 0. 03） with a flow rate of 0. 8 mL·min^-1. The detection wavelength was 210 nm. Result：The calibration curves was linear in the rang of 0. 8 -4. 0 μg, r = 0. 9995 and the resolution of betulinic acid was more than 1.5. The recovery rate was 102. 5% ,RSD =2.0%. Conclusion：This method is sensitive ,rapid and accurate with good reproducibility for quantitative analysis of betulinic acid in Semen Ziziphi Spinosae.