小麦TaLRK基因高效表达载体的构建及遗传转化

Construction of High-level Expression Vector with TaLRK Gene and Its Transformation in Wheat

从抗白粉病小麦(Triticum aestivumL)品系99/2439中分离到1个类受体蛋白激酶基因TaLRK。为了研究TaLRK基因对小麦白粉病菌[Blumeria graminis(DC)E O Speer fsptriticiEm Marchal]的抗性作用,以单子叶植物高效表达载体pAHC25为基础载体,将TaLRK基因插入到玉米泛素高效启动子Ubi1的下游,构建成pAH-TaLRK(+)植物高效表达载体。利用花粉管通道技术,以高产小麦新品种周麦18为受体进行了遗传转化。T0代植株经PPT抗性鉴定、PCR扩增检测,获得了19个转基因植株,为研究小麦TaLRK基因的功能奠定了基础。

Wheat （Triticum aestivum L）TaLRK gene, which encodes a receptor-like kinase, was isolated from a powdery mildew resistant wheat line, In order to investigate the resistance function of TaLRK gene against Blurneria graminis（DC） E O Speer fsp tritici Em Marchal （Bgt）, the TaLRK gene was inserted into the downstream of the high-effective promoter of maize （Zea mays） ubiquitin 1 to construct the plant high efficient expression vector pAH - TaLRK^（ ＋） based on a vector pAHC25 for monocotyledonous plant. A new high yield wheat cultivar ＂Zhoumai 18＂ was transformed with pAH- TaLRK^（＋） by pollen-tuber pathway and 19 positive transgenic plants of To generation were obtained by PPT - resistance screening and PCR detection, This study provided the good basic materials for gene functional study of TaLRK.