桑皮苷的镇咳平喘作用

Antitussive and antiasthmatic effects of mulberroside A

目的探讨桑皮苷镇咳、祛痰、平喘作用。方法采用小鼠浓氨水引咳法、小鼠SO2引咳法和豚鼠枸橼酸引咳法制造3种引咳模型,灌胃给予桑皮苷,以咳嗽潜伏期、咳嗽次数为评价指标,观察桑皮苷的镇咳作用;采用豚鼠磷酸组胺喷雾法制作引喘模型,以引喘潜伏期为评价指标,观察桑皮苷的平喘作用;采用小鼠酚红排痰法制作祛痰模型,以酚红排泌量为观测指标,观察桑皮苷的祛痰作用。结果桑皮苷80 mg.kg-1能明显抑制浓氨水引咳的潜伏期,桑皮苷80 mg.kg-1能明显抑制浓氨水引咳次数,桑皮苷20 mg.kg-1g能明显抑制SO2引咳次数,桑皮苷50 mg.kg-1能明显抑制枸橼酸引咳次数;桑皮苷100 mg.kg-1对组胺引起豚鼠支气管哮喘具有明显的平喘作用;桑皮苷质量浓度提高到160 mg.kg-1,对小鼠酚红排出量仍无影响。结论桑皮苷具有镇咳、平喘作用,但无祛痰作用。

Objective To investigate the antitussive, antiasthmatic and expectorant effects of mulberroside A, a major constituent of Cortex Mori. Methods Cough models were induced by ammonia, SO2 and citric acid in mice and guinea pigs respectively; The latency and number of cough were recorded to indicate the effect of mulberroside A. Histamine was used to induce the asthma model in guinea pigs and the latency of asthma was recorded as the indicator of the efficacy. The excretory amount of sputum was determined by phenolsulphonphthalein excretion in mice, which were indicated by the OD values. Results The latency of cough induced by ammonia in mice was significantly prolonged by mulberroside A. Numbers of cough were significantly diminished by mulberroside A 80 mg. kg^-1 in ammonia induced mice model, 20 mg. kg^-1 in SO2 induced mice model, 50 mg. kg^-1 in citric acid induced model of guinea pigs. However, the expectorant effect of mulberroside A had no significant change even at high concentration 160 mg. kg^-1. Conclusions Mulberroside A has significant antitussive effect and weak antiasthmatic effect, but no expectorant effect.