FQ-PCR检测乙型肝炎病毒DNA的临床价值

Real-time fluorescence quantitative PCR in determination of hepatitis B virus DNA

目的探讨荧光定量PCR(FQ-PCR)检测HBV-DNA的应用价值。方法从1000份用酶联免疫吸附试验(ELISA)检测乙肝五项的体格检查血清标本中,抽取288份HBsAg阳性标本及100份全阴标本,进行荧光定量聚合酶链式反应HBV-DNA定量分析。结果经FQ-PCR检测,80份HBsAg、HBeAg、HBcAb均阳性的标本,HBV-DNA阳性率为100%(80/80),平均HBV-DNA拷贝数为2.5×108拷贝/m l;164份HBsAg、HBeAb、HBcAb均阳性的标本,HBV-DNA阳性率为76.2%(125/164),平均HBV-DNA拷贝数为1.5×106拷贝/m l;12份HBsAg单项阳性的标本,HBV-DNA的阳性率为83.3%(10/12),平均HBV-DNA拷贝数为1.6×105拷贝/m l;100份HBsAg、HBsAb、HBeAg、HBeAb、HBcAb均阴性的标本,HBV-DNA的阳性率为2.0%(2/100),平均HBV-DNA拷贝数为4.5×105拷贝/m l。结论FQ-PCR可以检测HBV的感染和复制情况,对准确报告HBV感染、指导其选择治疗方案和观察疗效具有重要的临床意义。

Objective To explore the effect using fluorescence quantitative PCR （FQ-PCR） in detection of HBV-DNA in physical examination. Methods FQ-PCR was used to measure HBV-DNA of 288 HBsAg ＋ serum samples and 100 normal samples of 1000 serum samples tested by ELISA method in physical examination. Results Eighty HBsAg ＋/HbeAg ＋/HbcAb ＋ samples were all positive results （ 80/80）, with 2.5×10^8cp/ml of HBV- DNA on average. In 164 HBsAg ＋/HBeAb ＋/HBcAb ＋ samples, the average copy num- ber of HBV-DNA was 1.5 ×10^5cp/ml with a positive rate of 76.2% （125/164） . In 12 HBsAg ＋ samples, the average was 1.6 × 10^5cp/ml with a positive rate of 83.3% （10/12）. In 100 normal samples, the average was 4.5×10^5cp/ml with a positive rate of 2.0% （2/100）. Conclusion FQ-PCR was accurate for detection of HBV and can be used for monitoring the actual state of HBV infection and replication.