摘要
目的比较两种方法检测丙型肝炎病毒核酸(HCV-RNA)的结果。方法逆转录套式定性PCR(RT-nestedPCR)和荧光定量PCR对288例抗-HCV阳性的丙型肝炎患者血清标本进行HCV-RNA检测。结果288例抗-HCV阳性血清标本有248例RT-nested-PCR结果阳性,阳性率为86.1%(248/288);226例荧光定量PCR检测HCV-RNA>103拷贝/ml,检出率为78.5%(226/288),两种检测结果的阳性符合率为85.9%(219/255)。同时,29例标本定性结果阳性,定量结果<103拷贝/ml;7例标本定性结果阴性,定量结果>103拷贝/ml,差异具有统计学意义(χ2=12.25,P<0.01)。结论两种方法检测HCV-RNA,可以提高检测的灵敏度,为临床治疗提供依据。
Objective The primary outcome of this study was to compare the two methods which were used to detect serum hepatitis C virus RNA. Methods Serum HCV RNA of 288 patients with positive anti-HCV was measured by RT-nested PCR and fluorescence quantified PCR respectively. Results Among 288 patients, 248 were detected HCV RNA positive (86.5%) and 226 were measured HCV RNA 〉 10^3 copies/ml (78.5%). The coincidence rate of the two methods was 85.9%(219/255). Of the others, 29 patients with positive HCV RNA were measured HCV RNA 〈 10^3 copies/ml and 7 patients with negative HCV RNA were measured HCV RNA 3 〉 10^3 copies/ml. The difference was significant (χ^2 = 12.25, P 〈 0.01). Conclusion The two methods together to detect HCV RNA can improve the sensitivity, which provide the basis for clinical treatment.
出处
《国际检验医学杂志》
CAS
2006年第6期489-490,共2页
International Journal of Laboratory Medicine
关键词
肝炎
丙型
RNA
病毒
逆转录聚合酶链反应
荧光定量聚合酶链反应
Hepatitis C
RNA, viral
Reverse transcriptase polymerase chain reaction
Fluorescencequantitative polymerase chain reaction