内源性血管生成抑制因子Arresten原核表达载体的构建及表达

Plasmid construction of endogenetic angiogenesis inhibitor Arresten gene and its expression in E.coli DH5α

目的构建内源性血管生成抑制因子Arresten基因的原核表达载体,并在E.coliDH5α进行表达。方法从健康产妇的胎盘组织中提取总RNA,经逆转录—聚合酶链式反应(RT-PCR)扩增出Arresten基因,构建重组质粒pBV220-Arr转化E.coliDH5α进行原核表达。结果成功筛选出Arresten基因并构建了重组质粒pBV220-Arr,重组质粒在菌株中获得表达。结论构建的原核表达重组体pBV220-Arr能高效表达重组Arresten蛋白。

Objective To construct prokaryotic expression vector for endogenetic angiogenesis inhibitor Arresten gene, and to express recombinant in E, coli DH5α. Methods The total RNA was extracted from the placenta tissue of the normal puerperal, human Arresten gene was amplified by RT-PCR. The recombinant plasmid pBV220-Arr was transformed into E. coli DH5α after being constructed and expressed by this prokaryotie-expression system. Results The Arresten gene was screening successfully, and the recombinant plasmid pBV220-Arr was constructed successful. At the meantime pBV220-Arr was expressed in the DH5α. Conclusion The pBV220 - Arr can highly express Arresten proteinum.