人宫颈病变组织中HPV16 cDNA,HPV6/11,HSV-Ⅱ,CMV病毒蛋白的表达及对HPV16E7的影响

Expression of human papillomavirus16 cDNA and HPV6/11,HSV-Ⅱand CMV proteins in cervical lesions

目的探讨HPV16 cDNA,HPV16E7、HPV6/11、HSV-Ⅱ、CMV在各类宫颈病变中的表达及其在宫颈癌变中的作用。方法采用免疫组化方法对10例慢性宫颈炎、10例宫颈尖锐湿疣、45例宫颈上皮内瘤变、20例宫颈浸润癌、10例正常宫颈标本行HPV6/11、HSV-Ⅱ、CMV的测定。用原位杂交行HPV16CDNA的测定。结果HPV6/11在宫颈尖锐湿疣组阳性表达率明显增高(P<0.05)。HPV16 cDNA,HSV-Ⅱ及CMV在宫颈癌及宫颈上皮内瘤变中的阳性表达率均增高(P<0.05)。随病变进展HPV16 cDNA表达增加,与宫颈癌及宫颈上皮内瘤变呈正相关。HPV16 cDNA在宫颈癌及宫颈上皮内瘤变中的阳性表达率明显高于HSV-Ⅱ、CMV的表达(P<0.05)。在宫颈癌及宫颈上皮内瘤变时,HPV16E7在HPV16合并HSV-Ⅱ及CMV感染时阳性表达率高于单纯HPV16感染时的表达。结论HPV6/11是引起宫颈癌和尖锐湿疣的主要病因。HSV-Ⅱ及CMV在宫颈癌的发生发展过程中与HPV有一定的协同作用。

Objective To study the expression of HPV16 cDNA and HPV6/11, HSV-Ⅱ and CMV in Cervical Lesions and function in the cervical neoplasma generation. Methods HPV6/11, HSV-Ⅱ and CMV of 10 cases of nomal cervix, 10 chronic cervicitis, 10 cervical eondyloma,45 CINs and 20 cervical carcinoma were detected by the immunohistochemistry, and HPV16 cDNA by in situ hybridization. Results Cervical condyloma showed a sigificantly higher HPV6/11 infection rate. Cervical carcinoma and CINs showed sigificantly higher HPV16 cDNA, HSV-Ⅱ and CMV infection rates than the nomal cervix, chronic cervicitis and cervical condyloma（P〈0.05）, the expression of HPV16 cDNA increased following progress of cervical lesions. Between the expression of HPV16 cDNA and cervical carcinoma, CINs there was positive correlation（ P〈0.01）. Cervical carcinoma CINs show a sigificantly higher HPV16 cDNA infection rate than HSV-Ⅱ and CMV（ P〈0.05）, when HPV16 infection cervical carcinoma and CINs emerged with HSV- Ⅱ and CMV, the expression rate of HPV16E7 was higher than that of HPV16E7 when only infection of HPV16. Conclusion HPV16 is main reason for the cervical carcinoma. HPV6/11 is main reason for the cervical condyloma. HSV-Ⅱ, CMV and HPV16 can act together to cervical carcinoma＇ s generation.