p16蛋白在预测尖锐湿疣癌变中的应用

Predicting cancerization of condyloma acuminatum by testing expression of p16

目的探讨p16基因的表达产物—p16蛋白在早期诊断和预测尖锐湿疣癌变中的价值。方法应用免疫组化法检测p16蛋白在15例正常皮肤黏膜、35例尖锐湿疣、12例尖锐湿疣合并非典型增生以及3例尖锐湿疣癌变组织中的表达情况,同时用荧光定量PCR(FQ-PCR)方法检测上述相同组织中HPV6/11、HPV16/18 DNA。结果正常皮肤组与尖锐湿疣组比较,p16蛋白表达的差异有显著性(P<0.05);正常皮肤组与尖锐湿疣合并非典型增生及癌变组比较,p16蛋白表达的差异有显著性(P<0.01);尖锐湿疣组与尖锐湿疣合并非典型增生及癌变组比较,p16蛋白表达的差异有显著性(P<0.01)。结论尖锐湿疣患者p16蛋白的强阳性表达提示有癌变的可能,检测p16蛋白可能是早期诊断和预测尖锐湿疣癌变的方法之一。

Objective To investigate the value of the over-expression of p16 protein in predicting early caneerization of CA. Methods p16 protein was detected with immunohistochemical SP method in the lesions of 15 cases of nomal skin or museosa,35 cases of eondyloma acurninata（CA）, 12 cases of CA accompanied dysplasis and 3 cases of cancerization of CA. HPV6/11, HPV16/18 DNA were examined with FQ-PCR method in the same specimens. Results The expression of p16 protein was different between nomal skin and CA（P〈0.05）; The expression of p16 protein from CA accompanied dysplasis and caneerization of CA was markly higher compared to nomal skin or muscosa（P〈 0.01 ）. The expression of p16 protein from CA accompanied dysplasis and cancerization of CA was marldy higher compared to CA（P 〈 0.01 ）. Conclusion The over-expression of p16 protein in CA tissue implied the risk of cancerization of CA, which might be a useful method for predicting cancerization of CA.