食品中邻苯二甲酸酯的二极管阵列检测高效液相色谱测定法

Diode Array High Performance Liquid Chromatography in Determining Phthalic Acid Esters in Foods

目的建立一种同时测定食品中5种邻苯二甲酸酯类（PAEs）残留的方法，调查这5种物质在食品中的污染情况。方法用正己烷浸泡，超声提取对样品中PAEs进行提取并净化，采用反相高效液相色谱-二极管阵列检测法测定其中邻苯二甲酸二甲酯（DUP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸二异辛酯（DEHP）、邻苯二甲酸二正辛酯（DOP），并用建立的方法分析实际样品。结果5种PAEs线性相关系数r〉0．997，RSD在1．2％-9．3％之间，高、中、低不同水平的加标回收率在80．9％～119．8％之间，测定38种食品样品PAEs的检出率为18．4％。结论该方法简便快速，精密度较高，重现性较好，可应用于食品中5种邻苯二甲酸酯类的同时测定。调查结果显示，食品中邻苯二甲酸酯类环境激素的污染比较严重，应引起有关部门的重视。

[ Objective ] To establish a method for determining 5 kinds of phthalic acid esters （ PAEs ） in foods. [ Methods ] Samples were marinated in hexane to extract and purify PAEs; reverse phase high performance liquid chromatography was applied to determine and analyze DMP, DEP, DBP, DEHP and DOP in the samples. [Results]The correlation coefficients of the 5 kinds of PAEs were above 0.997; the relative standard deviation （RSD） ranged from 1.2% to 9.3% ; the recoveries were between 80.9% and 119.8% ; the detection rate was 18.4%. [Conclusion]This method is simple and accurate; it can be applied to the determination of PAEs in foods. The pollution of phthalate is quite serious, great importance should be attached to this problem.