摘要
目的利用噬菌体展示随机肽库技术,进行NMDAR1抗原表位研究。方法以R1JHL单克隆抗体作为筛选分子,生物淘洗噬菌体展示随机12肽文库。经过4轮的筛淘,ELISA结合实验鉴定阳性克隆,提取阳性克隆噬菌体单链DNA进行序列分析。结果对阳性噬菌体进行递呈肽氨基酸序列分析,获得一阳性克隆序列HYNLSSDRKVRL,与R1JHL单克隆抗体识别序列相比,两者存在一致性序列DRK。结论DRK可能为NMDA受体抗原表位。
Objective To select minotope of NMDAR1 by using phage displayed random peptide library. Methods R1JHLmcAb was used as target to screen the binding peptide from a 12-mer Ph. D. random peptide library. After four rounds of bipanning, the peptide sequence of positive clones was determined and analyzed by DNA sequencing and ELISA assay. Results A positive clone was found （HYNLSSDRKVRL）. There was a consentient sequence DRK in NMDAR1. Conclusion DRK in NMDAR1 might be the B cell epitope recognized by R1JHLMeAb.
出处
《河北医药》
CAS
2006年第5期343-344,共2页
Hebei Medical Journal
关键词
噬菌体随机肽库
单克隆抗体
表位
NMDA受体
phage-displayed random peptide library
monoclonalantibody
epitope
NMDA receptor
