缬沙坦、灯盏花素对1型糖尿病大鼠破骨细胞的影响

The effect of valsartan and Dengzhanhuasu on osteoclast in type 1 diabetic rats in vitro

目的研究体外培养的1型糖尿病大鼠破骨细胞的特点及缬沙坦、灯盏花素对破骨细胞的影响,探讨1型糖尿病骨丢失的机制。方法以链脲佐菌素(STZ)制成1型糖尿病大鼠模型并用缬沙坦、灯盏花素进行干预,实验分为正常对照组、1型糖尿病组、缬沙坦组、灯盏花素组。于制成模型后4周进行破骨细胞体外培养。结果1型糖尿病组和缬沙坦组大鼠体外培养的破骨细胞数量多于正常对照组,1型糖尿病组和缬沙坦组之间无差异;灯盏花素组体外培养的破骨细胞数量与正常对照组比较差异无显著性,但少于同期1型糖尿病组与缬沙坦组。结论1型糖尿病时破骨细胞数量增加,导致骨吸收大于骨形成,可能是1型糖尿病骨丢失的发病机制。灯盏花素治疗能影响体外培养的破骨细胞数量。

Objective To investigate the characteristics of cultured osteoclast in type 1 diabetic rats and the effect of valsartan and Dengzhanhuasu on osteoclastin, and to explore the possible mechanism of bone loss. Methods The diabetic model in male Sprague-Dawley rats was made by injection of streptozotocin （STZ）.The diabetic rats were divided into 4 groups： control group, model group, valsartan treatment group and Dengzhanhuasu treatment group. The osteoclasts were cultured 4 weeks after the diabetic model was made. Results At the 4th week, the number of osteoclast was increased in model group and valsartan treatment group, as compared with that in control group. There was no significant difference between model group and valsartan group. The number of osteoclast in Dengzhanhuasu treatment group was lower than that in model group and valsartan treatment group. Conclusion The increase of osteoclasts in type 1 diabetes mellitus could result in bone loss. Dengzhanhuasu and valsartan could influence the number of osteoclasts cultured in vitro.