瓜类枯萎病菌粗毒素的致萎作用及其钝化研究

Wilting Effect of Gourd Fusarium Wilt Pathogen and Its Inaltivation

通过试验对瓜类枯萎病菌进行了产毒培养基的筛选,测定不同枯萎病菌菌株产毒能力,以及12种化合物对甜瓜枯萎病菌毒素的钝化作用。研究结果表明:改良的理查培养液最适于枯萎病菌的生长,其粗毒素对幼苗的致萎作用最强,并随着处理浓度的增加呈规律性变化;不同枯萎病菌菌株粗毒素对甜瓜幼苗的致萎作用差异显著;KMnO4、ZnSO4、VB6、VB1、CuSO4、CaSO4和MnSO4等均可不同程度地钝化甜瓜枯萎病菌毒素使其毒性降低。

Four kinds of culture media for gourd Fusarium wilt pathogen were used for gourd Fusarium wilt pathogen to produce toxin and test the capability of different strains for producing toxin. Twelve compounds were also tested for inactivating the toxin of Fusarium wilt pathogen. The results was indicated that the improved Czapek culture medium was the most favorable for the pathogen growing, and its toxin had the strongest inhibition effect on seedling grow. With the increase of toxin concentration, the inhibition effect changed regularly. Different strains have a remarkable difference of inhibition melon seedling growth. The compounds, KMnO4 , ZnSO4 , VB6 , VB1 , CuSO4, CaSO4 and MnSO4 could inactivate the toxins of the pathogen in various degree.