辽宁地区油松松针枯萎病病原及其培养特性

Pathogentic Fungus and Pure Culture of Withered Disease of Chinese Pine

对辽宁地区油松枯萎病的病原菌及培养特性进行了研究。通过广泛标本采集和分类鉴定，确定油松上5种病害。接种试验证明松落针病[Lophodermium conigenum（Brunaud）Hilitz]、松赤枯病（Pestalotia funerea Desm．）和松叶枯病（Sphaeropsis sp）3种病原有明显致病性。3种病原菌在36h时孢子萌发率达到99％以上。菌丝的最佳生长温度在20～30℃之间，最佳碳源是果糖和蔗糖，最佳pH值6～8。

This paper reports the reseach of the pathogentic fungus and the pure culture characteristic of Chinese pine withering disease in Liaoning. Through widespread specimen collecting and classified appraisal, 5 kind of fungal disease on leaves of Chinese pine had been definited. The vaccination experiment had proven obvious pathogenicity of pine cast needle { Lophodermium conignum （ Brunaud ） Hilitz ） , pine red leaf sickness （ Pestalotia funerea Desm. ） and pine needle blast （ Sphaeropsis sp ） to Chinese pine. Spore sprouting rate of three kinds of pathogentic fungus were above 99% in 36 hours. The best temperature for hypha growth was from 20 to 30℃; The best carbon source was fructose or sucrose, and the best pH was from 6 to 8.