摘要
对辽宁地区油松枯萎病的病原菌及培养特性进行了研究。通过广泛标本采集和分类鉴定,确定油松上5种病害。接种试验证明松落针病[Lophodermium conigenum(Brunaud)Hilitz]、松赤枯病(Pestalotia funerea Desm.)和松叶枯病(Sphaeropsis sp)3种病原有明显致病性。3种病原菌在36h时孢子萌发率达到99%以上。菌丝的最佳生长温度在20~30℃之间,最佳碳源是果糖和蔗糖,最佳pH值6~8。
This paper reports the reseach of the pathogentic fungus and the pure culture characteristic of Chinese pine withering disease in Liaoning. Through widespread specimen collecting and classified appraisal, 5 kind of fungal disease on leaves of Chinese pine had been definited. The vaccination experiment had proven obvious pathogenicity of pine cast needle { Lophodermium conignum ( Brunaud ) Hilitz ) , pine red leaf sickness ( Pestalotia funerea Desm. ) and pine needle blast ( Sphaeropsis sp ) to Chinese pine. Spore sprouting rate of three kinds of pathogentic fungus were above 99% in 36 hours. The best temperature for hypha growth was from 20 to 30℃; The best carbon source was fructose or sucrose, and the best pH was from 6 to 8.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2006年第2期204-208,共5页
Journal of Shenyang Agricultural University
基金
"九五~十五"辽宁省科技攻关课题(98207002)
关键词
油松
枯萎病
病原
培养特性
接种试验
withered disease of Chinese pine
path ogentic fungus
pure culture