枳壳超微粉甲醇提取物的高效液相指纹图谱初探

HPLC fingerprint of Fructus aurantii micropowder by methanol extraction

目的：建立枳壳超微粉甲醇提取物的高效液相指纹图谱（HPLC-FP），为枳壳超微粉的鉴定提供实验依据。方法：采用计算机辅助相似性评价系统，对17种不同购买地的枳壳粗粉及超微粉进行相似度评价，并比较传统粗粉与超微粉的相关性。结果：枳壳超微粉中柚皮苷、橙皮苷、新橙皮苷的溶出较粗粉高，两者甲醇提取物的相似度在0．90-1.0。结论：该色谱图可作为枳壳超微粉甲醇提取物的HPLC-FP，用于枳壳超微粉甲醇提取物的质量评价，并进一步证实超微粉碎更有利于黄酮类成分的溶出，且超微粉碎后枳壳中甲醇提取物无质的改变，仅属物理变化。

AIM： To provide a HPLC fingerprint of Fructus aurantii micropowder in order to create a basis for identification. METHODS： With the help of computer similarity evaluation, Seventeen kinds of Fructus aruantii sample were pulverized and micronized separately, then were extracted by methanol and compared correlation between both extracts. RESULTS： In methanol extraction, contents of aurantiamarin, hesperidin and neohesperidin in micropowder were more than that in pulverized powders, correlation of both HPLC fingerprint was 0.9 -1.0. CONCLUSION： The method established can be used for identifying and evaluating crud drug of Fructus aurantii, and further prove that micronization is beneficial to increasing flavonoid dissolution.