新疆桑叶中挥发油化学成分的GC/MS分析

Chemical component of essential oil in Mulberry Leaves by GC-MS

目的：建立一种新疆桑叶中挥发油的化学成分分析的方法。方法：采用乙醚超声萃取-水蒸气蒸馏的方法提取桑叶中的挥发油，用气相色谱．质谱法对桑叶中挥发油的化学成分进行了分析。共分离出80余峰，鉴定出了55种物质，占挥发油总成分的90％以上。用面积归一化法确定了各组分的相对含量。结果：所分离出的化合物中含有烷烃（C10-C40）、烯烃、不饱和醇、不饱和醛、不饱和酮、羧酸、酯、杂环化合物及芳香族化合物。主要成分为正十八烷（9．11％）、邻苯二甲酸二（2-甲基）丙酯（8．92％）和3，7，11，15-四甲基-2-十六碳烯-1-醇（7．19％）。挥发油中还含有少量的萜类化合物。结论：本方法稳定可靠，重现性好，适用于中药挥发油的化学成分分析。

AIM ： To establish a method of analysing chemical components of the essential oil in Mulberry Leaves in Xinjiang. METHODS： The essential oil of Mulberry Leaves was extracted by ultrasound-steam distillation method, components were measured by GC-MS. About 80 peaks were separated totally. 55 components of which were identified. The amount of the components from the essential oil was determined by normalization method. RESULTS： There were alkane（C10 - C40）, alkene, alcohols, unsaturated aldehydes, unsaturated ketones, carboxylic acid, ester,heterocyclic compound and aromatic hydrocarbon in essential oil in Mulberry Leaves. The main components were octadecane （9.11% ）, 1,2-benzenedicarboxylic acid-his （ 2-methyl ） propyl ester （ 8.92% ）, 3,7, 11,15-tetramethyl-2-hexadecen-l-ol （7.19%）. there was a small amount of terpene in essential oil in Mulberry Leaves. CONCLUSION： The method is reliable, stable and has a good repeatability. This method can be applied to the analysis of the essential oil components extracted from Chinese traditional medicine.