分光光度法测定dl─HMPAO冻干药盒中微量Sn（Ⅱ）

SPECTROPHOTOMETRIC DETERMINATION OF MICROAMOUNTS OF Sn(Ⅱ) IN dl HMPAO KIT

采用分光光度法及Ｓｎ（Ⅱ）－Ｆｅ（Ⅲ）－邻二氮菲（Ｐｈｅｎ）体系测定ｄｌ－ＨＭＰＡＯ冻干药盒中微量Ｓｎ（Ⅱ）。在ｐＨ≈５的酸度下，Ｓｎ（Ⅱ）还原Ｆｅ（Ⅲ）生成红色的Ｆｅ（Ⅱ）－Ｐｈｅｎ配合物。该配合物的最大吸收波长λｍａｘ＝５１０ｎｍ，计算得到表观摩尔吸光系数为２．０×１０４ｌ·ｍｏｌ－１·ｃｍ－１。Ｓｎ（Ⅱ）浓度在０－２．５μｇ／ｍｌ内符合比尔定律。

A spectrophotometric determination of microamounts of Sn(Ⅱ) in dl HMPAO kit is described, with ferric chloride hexahydrate 1, 10 phenanthroline as chromogenic reagent is studied. The complex is formed at pH 5, the apparent molar absorptivity is 2.0×10 4 l·mol -1 ·cm -1 at 510 nm. Beers law is obeyed in the range of 0-2.5 μg/ml for Sn(Ⅱ).