慢性乙肝病毒携带者外周血T细胞亚群的变化及其与血清HBVDNA的关系研究

Association of the chang of T cell subset with HBV DNA levels in chronic hepatitis B-virus carriers

目的研究慢性乙肝病毒携带者（AsC）的细胞免疫功能变化及临床意义，探讨其与血清HBVDNA的关系。方法对120例慢性乙肝病毒携带者和60例健康人应用流式细胞仪直接免疫荧光法检测外周血T淋巴细胞亚群的百分率，并进行比较。结果乙肝病毒携带者外周血CD3^＋、CD4^＋细胞数百分率及CD4／CD8与健康对照组比较明显降低（P〈0．01）；CD8^＋细胞数百分率较健康对照组明显升高（P〈0．01）；血清HBVDNA（＋）组与HBVDNA（-）组相比，CD3^＋细胞数百分率无统计学差异（P〉0．05），CD4^＋细胞数百分率及CD4^＋／CD8^＋比值明显降低（P〈0．01），CD8^＋细胞数百分率明显升高（P〈0．01）。将HBVDNA（＋）组按高、中、低病毒载量分组进行比较，各项指标均无统计学意义（P〉0．05）。但CD4^＋、CD4^＋／CD8^＋在数值上呈下降趋势，CD8^＋呈上升趋势。结论HBV感染后可导致乙肝病毒携带者细胞免疫功能降低，HBVDNA复制进一步加重紊乱。

Objective To study the significance of the change of T cell subsets and find out the influence of HBV DNA levels on function of cellular immunity in hepatitis B-virus carders. Methods Peripheral T cell subsets in 120 chronic HBV carriers and 40 health control groups were determined by direct immunofluorescence using flow cytometry. Result The rate of CD3^＋ cell, CD4^＋ cell and CD4/CD8 were significantly reduced （P〈0.01 ）, and CD8^＋ cell was significantly increased （P〈0.01 ） in 120 symptomatic HBV carriers as compared with health control groups. The rate of CD3^＋ cell in HBV DNA （＋） carriers showed no significant difference from those of HBV DN （-） carders. The rate of CD8^＋ cell was significantly increased （P〈0.0 1） as compared with HBV DNA （-） carriers. There was no significantly difference as compared with difference HBV DNA levels, but the rate of CD4^＋ cell and CD4/CD8 were lower, CD8^＋ cell was higher with increasing levels of HBV DNA in HBV DNA （＋） carriers. Conclusions These findings suggest that the disorder of cellular immunity in patients with hepatitis B would be caused by HBV infected, and the increasing of HBV DNA in subset can make the disorder advanced.