3种肺炎克雷伯菌生物膜制备方法的比较

Comparison of three methods for preparing the biofilm produced by Klebsiella pneumoniae

目的比较3种体外制备肺炎克雷伯菌生物膜的方法。方法用高分子滤膜琼脂培养法、高分子滤膜肉汤培养法和输液管肉汤培养法制备肺炎克雷伯菌生物膜,通过菌落计数判断3种方法的效果。结果培养5和10d后,3种方法的菌落计数分别是:(1.32±0.26)×109CFU/mL,(0.22±0.026)×109CFU/mL,(0.15±0.025)×109CFU/mL和(3.68±0.51)×109CFU/mL,(0.24±0.022)×109CFU/mL,(0.20±0.035)×109CFU/mL。高分子滤膜琼脂培养法明显优于其他2种方法(P<0.01)。结论用高分子滤膜琼脂培养法制备肺炎克雷伯菌生物膜操作简便,效果最佳。

Objective To compare three methods for preparing the biofilm of Klebsiella pneurnoniae in vitro. Methods K. pneurnoniae biofilm was prepared through high molecular membrane agar method, high molecular membrane broth method and infusion tube broth method. The number of colonies in biofilm was counted to judge the utility of each method. Results The colony count of above three methods were （1.32 ± 0.26） ×10^9CFU/mL, （0.22 ± 0. 026） ×10^9CFU/mL and （0.15 ± 0. 025） ×10^9 CFU/mL respectively after 5 days incubation and （3.68 ± 0.51 ） ×10^9CFU/mL, （0. 24 ± 0. 022） ×10^9 CFU/mL and （0.20 ± 0. 035） ×10^9CFU/mL after 10 days. High molecular membrane agar method was much better than the other two methods （P 〈0.01 ）. Conclusions High molecular membrane agar method is a convenient and effective method to prepare K. pneurnoniae biofilm.