大肠癌组织大肠癌缺失基因杂合缺失的研究

STUDY ON THE LOSS OF HETEROZYGOSITY AT THE DCC LOCUS IN COLORECTAL CANCER

采用聚合酶链反应（ＰＣＲ）和限制性片段长度多态性分析（ＲＦＬＰ）技术，对４ｌ例大肠癌组织大肠癌缺失基因杂合性缺失进行研究。结果表明，３８例大肠癌信息个体中有２１例（５５．３％）发现ＤＣＣ基因杂合缺失。ＤＣＣ基因杂合性缺失与肿瘤大小、组织学类型和浸润深度无关，但有淋巴结转移（８０．００％）和ＤｕｋｅｓＣ、Ｄ期（７１．４％）大肠癌Ｄｃｃ基因杂合缺失率显著高于无淋巴结转移（３９．１％）和ＤｕｋｅｓＡ、Ｂ期组（３５．３％，Ｐ＜０．０５）。结果提示，ＤＣＣ基因杂合缺失多发生于大肠癌的晚期，ＤＣＣ基因杂合性缺失的检测有可能成为大肠癌病人预后评价的指标。

Loss of heterozygosity(LOH)at the DCC gene was analyzed in 4l cases of colorectal carcinomas by polymerase chain reaction-restriction fragment length polymorphims(PCR-RFLP).LOH at the DCC locus bad no relation to tumor size,histologicaI type,and the depth of tumor invasion,whereas the incidence of LOH at DCC locus in colorectal carcinomas was significantly higher in lymph-node metastases positive (80.O%)and Duckes C,D stage groups ( 7l.4%)than in lymph-node metastases negative ( 39.1%)and A,B stage groups (35.3%,P<0.05).Our results suggested that LOH at DCC gene occurs at advanced stage of colorectal carcinoma,and that the determination of LOH at DCC locus may be useful for predicating the prognoisis of colorectal Carcinoma.