流式细胞术检测HLA-B27抗原的表达及意义——与微量淋巴毒实验的比较

Significance and detection of expression on HLA-B27 antigen by flow cytometry:comparison of microlymphocytotoxicity test

目的对不同HLA-B27检测方法之间的差异及各自的优缺点进行评价并探讨其临床意义。方法分别采用微量淋巴毒法(MCLT)及流式细胞术(FCM)检测134例患者外周血标本的HLA-B27抗原,将二者结果进行分析比对;并对患者人群中的B27荧光强度与发病年龄及患病时间之间进行相关分析。结果两种检测方法检测脊柱关节病组和对照组HLA-B27阳性例数及阳性率相比,差异均无统计学意义,结果符合率为94.8%,仅有7例不符合,而对照组的符合率为100%;B27荧光强度与起病年龄之间存在较弱的正相关(r=0.345,P=0.026),而与患病时间之间存在较弱的负相关(r=0.371,P=0.006)。结论两种检测方法均准确可靠,HLA-B27检测对AS的早期诊断与判断预后意义重大;B27表达强度与发病年龄及患病时间之间可能存在一定程度的关联。

Objective To evaluate the differences, advantages and disadvantages of serology microlymphocytotoxicity test （MCLT） and flow cytometry（FCM） for detecting A-B27, and to investigate the clinical significance of detection on HLA-B27. Methods A total of 134 samples from patients were entered into the study, two methods （MCLT and FCM） were used to detect the expression of HLA-B27 antigen, and the results were analyzed ; meanwhile the relationship between the intensity of B27 expression,occurrence age of patients and course of disease. Results The coincide rate of positive HLA-B27 of two methods was 94.8%, only 7 cases had different results, however the coincide rate of positive HLA-B27 of two methods was 100% in control, meanwhile the intense of B27 expression had a weak positive correlation with occurrence age of patients（r= 0. 345, P=0. 026）,and it had a weak negative correlation with course of disease（r=0.371,P= 0. 006）. Conclusion MCLT and FCM prove to be accurate and reliable techniques, suitable for clinical application：Detection of HLA-B27 has a remarkable clinical significance on early diagnos is and prognosis of ankylosing spondylitis. There is the possible correlation between the intensity of B27 expression and occurrence age of patients, and course of disease.