Naphtho-[2,3-b]pyrandiones类抗生素SP-1的分离与结构研究

Isolation,purification and structural identification of SP-1,a naphtho-[2,3-b]pyrandiones group antibiotics

在利用化学筛选法获得新染料类抗生素研究中,从产生菌Z-2002中,应用硅胶柱层析、薄层层析、凝胶柱层析及重结晶,分离纯化得到化合物SP-1,该化合物分子式为C_(14)H(12)O_6,分子量为276。SP-1与浓硫酸反应,呈鲜红色,对金黄色葡萄球菌209P有明显的抑制作用。通过UV、IR、EI-MS、HR-MS、~1H-NMR、^(13)C-NMR、DEPT、~1H-~1H COSY、”C一。H COSY、HMBC、比旋光度等光谱学数据分析,确定了SP-1的结构,证明该化合物与(+)-Cryptosporin一致并首次利用2D核磁共振数据明确了(+)-Cryptosporin结构中各个~1H、^(13)C信号的归属。

During the screening for new antibiotics, a naphtho-[2, 3-b] pyrandiones group antibiotic SP-1 was purified by silica gel column and sephadex LH-20. HPLC analysis showed that the purity of SP-1 is higher than 97%. The SP-1 can be crystallized with CH30H as yellow crystal. Chemical structure of SP-1 was identical with cryp- tosporin after careful spectral analysis based on UV, IR, EI-MS, HR-MS, ^1H-NMR, ^13C-NMR, DEPT, ^1H-^1H COSY, ^13C-^1H COSY, HMBC. Its absoluted configuration has been confirmed by comparison of specific rotatory power with natural occurring （ ＋ ） -cryptosporin and chemical synthetic （-） -cryptosporin. SP-1 has activity against gram-positive bacteria.