原位沉析羟基磷灰石-壳聚糖骨组织工程支架材料的研制

Preparation of in situ-deposited hydrxyoapatite on chitosan scaffold

仿生构建羟基磷灰石-壳聚糖复合材料是制备骨组织修复材料的重要途径之一。本研究利用有机官能团对无机物矿化的调控作用,在壳聚糖多孔支架表面原位沉析羟基磷灰石(HAp)。在仿生溶液中,采用简单的化学处理,使HAp晶体在壳聚糖多孔支架表面原位沉析。研究结果表明壳聚糖分子结构中的氨基作为成核位点,在碱性条件下首先与吸附Ca2+,再通过静电作用力吸附仿生溶液中的PO43-、OH-等其它离子促使HAp晶体在壳聚糖支架材料表面的成核、长大。此类材料有望成为一种生物活性的骨组织工程材料。

Biomimetic growth of calcium phosphate over chitosan might be an effective approach to constitute inorganic-organic composite biomaterial. In this work,the in situ-deposition of hydroxyapatite on chitosan scaffold was studied via the effect of organic matrix on the mineralization of inorganic ions,in order to improve the biological feature of the scaffold. According to the result of XRD,SEM and EDX, HAP precipitated on the surface of the chitosan scaffold which was pre-treated by being rinsed in Ca（OH）2 saturation solution. Amidogen of chitosan acts as the nucleating site,which firstly absorb Ca^2＋ in basic solution,and then induce PO4^3- and OH^- by electrostatic interaction in 1.5 simulated body fluid while the concentration of ions for forming HAP are over its saturation at the area of amidogen, then HAP in situ formed on the surface of chitosan scaffold. This material was expected to be a bioactive bone tissue engineering material.