婴幼儿轮状病毒性肠炎血清中IL-2、IL-15及TNF-α含量的变化及临床意义

Serum levels of IL-2,IL-15,and TNF-α in infants with acute rotavirus enteritis

目的研究急性轮状病毒性肠炎患儿血清中IL-2I、L-15和TNF-α含量的变化,探讨它们在急性轮状病毒性肠炎中的作用。方法留取50例急性轮状病毒性肠炎(病程<4 d)患儿血清标本,留取同期21例正常儿童的血清作为正常对照。采用ELISA方法检测血清中IL-2I、L-15及TNF-α的含量。结果50例急性轮状病毒性肠炎患儿血清IL-2I、L-15及TNF-α含量均较对照组显著升高(P<0.001或P<0.005)。其中,IL-2含量与采血当日的大便量呈负相关(r=-0.286,P<0.05);呕吐组与未呕吐组比较IL-15含量明显降低(P<0.05);重型组与轻型组,发热组与未发热组比较TNF-α含量明显升高(P<0.05),TNF-α含量与腹泻次数呈正相关(r=0.373,P<0.01)。结论细胞因子IL-2、IL-15和TNF-α在轮状病毒感染的免疫发病机制中可能起重要作用,而且与临床症状及病情轻重有关。

Objective To explore the changes of serum interleukin-2（IL 2）,interleukin 15（IL-15）and tumor necrosis factor alpha（TNF-α） and their clinical significances in infants with acute rotavirus enteritis. Methods Serum was collected within four days after the onset of illness from 50 children with acute rotavirus enteritis. Another 21 normal children were studied as controls. The concentrations of IL-2, IL-15, and TNF-α in serum were estimated by the method of Enzyme-Linked Immunosorbent Assay（ELISA）, Results The concentrations of IL-2, IL-15, and TNF-α in serum of 50 patients with acute rotavirus enteritis were significantly higher than those in control group（P 〈 0. 001 or P 〈 0. 005 ）, There was a negative relation between the level of IL-2 and the daily quantity of stool （r = - 0. 286, P 〈 0.05 ）. The levels of serum IL-15 in patients with vomiting were significantly lower than those without vomiting （P 〈 0.05 ）. The levels of serum TNF-α were significantly higher in severe disease and fever group than those in mild disease and nonfever group （P 〈 0.05）. There was a positive relation between the level of TNF-α and the frequency of diarrhea（ r = 0. 373, P 〈 0.01） .Conclusion The levels of serum IL-2, IL-15, and TNF-α were obviously increased in infants with acute ro- tavirus enteritis and correlated with the degree of disease severity and clinical symptoms, and may play important roles in the immunopathogenetic mechanisms.